The UK Immunological Toolbox project at Pirbright has developed and validated a high throughput workflow for sequencing hundreds of hybridoma clones. The workflow employs a universal method that allows sequencing of any hybridoma clone. Just under 400 hybridomas producing antibodies against a range of host species and viral pathogens have been sequenced (cattle; 200, chicken; 100, viruses; 50 and pig;15).
A review of the past, present and future of the veterinary immunological toolbox has been recently published, with specific reference to recent developments discussed at the International Union of Immunological Societies (IUIS) Veterinary Immunology Committee (VIC) Immune Toolkit Workshop at the 12th International Veterinary Immunology Symposium (IVIS) in Seattle, USA. The article is available as open access on Frontiers in Immunology: